IGF1 Antikörper

Produktnummer ABIN211952

Produktdetails anti-IGF1 Antikörper

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IGF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Recognizes human IGF1. Cross-reactivity with IGF-II: ~40 % . Species cross-reactivity: mouse, rat and possibly chicken. Occasional difficulty neutralizing rat IGF1 due to binding protein interference.

Aufreinigung: Protein G purified

Immunogen: Human insulin-like growth factor I (IGF1) purified from human plasma.

Type of Immunogen: Purified protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), IP, Neut (10 - 20 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Neutralization and Immunohistochemistry. Immunoprecipitation: 5 μg immunoprecipitated 100 ng IGF1. Neutralization: 10-20 μg/mL can inhibit the activity of 10 ng/mL of IGF1 as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay. Immunohistochemistry: 10 μg/mL. Reported to detect IFG1 in formalin-fixed, paraffin-embedded skin sections.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 200 μL sterile ddH2O

Konzentration: Lot specific

Buffer: Supplied as a lyophilized powder from 0.1M Tris-glycine,  pH 7.4, 0.15  mM sodium chloride, 0.1  mM EDTA. No preservative added.

Konservierungsmittel: Without preservative

Handhabung: Avoid frequent freeze and thaws.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Details zu IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Andere Bezeichnung: IGF-I / IGF1 (IGF1 Produkte)

Hintergrund: Name/Gene ID: IGF1
Family: Hormone

Synonyms: IGF1, IGF-IA, IGF-IB, IGFI, IBP1, IGF-I, Insulin-like growth factor 1, Insulin-like growth factor I, Insulin-like growth factor IA, Insulin-like growth factor IB, Mechano growth factor, Somatomedin-C, IGF1A, MGF

Gen-ID: 3479

Pathways: RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation