PKC gamma Antikörper (AA 351-614)

Produktnummer ABIN2118965

Der Maus Monoklonal Anti-PKC gamma-Antikörper wurde für WB, IHC, IP und ICC validiert. Er ist geeignet, PKC gamma in Proben von Ratte zu detektieren.

Kurzübersicht für PKC gamma Antikörper (AA 351-614) (ABIN2118965)

Target: PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PKC gamma Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-PKC gamma Antikörper

Bindungsspezifität: AA 351-614

Verwendungszweck: Monoclonal Antibody to Protein Kinase C Gamma (PKCg)

Sequenz: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- FSFLMVLGKG SFGKVMLAER RGSDELYAIK ILKKDVIVQD DDVDCTLVEK RVLALGGRGP GGRPHFLTQL HSTFQTPDRL YFVMEYVTGG DLMYHIQQLG KFKEPHAAFY AAEIAIGLFF LHNQGIIYRD LKLDNVMLDA EGHIKITDFG MCKENVFPGS TTRTFCGTPD YIAPEIIAYQ PYGKSVDWWS FGVLLYEMLA GQPPFDGEDE EELFQAIMEQ TVTYPKSLSR EAVAICKGFL TKHPGKRLGS GPDGEPTIRA HGFF

Spezifität: The antibody is a mouse monoclonal antibody raised against PKCg. It has been selected for its ability to recognize PKCg in immunohistochemical staining and western blotting.

Kreuzreaktivität: Human, Maus

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PKCg (AA 351-614)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu PKC gamma

Target: PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

Andere Bezeichnung: PKCg

Hintergrund: PKC-G, PRKCG, PKCC, SCA14, PKC-Gamma

Pathways: WNT Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, G-protein mediated Events, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGF Signaling