p21 Antikörper

Produktnummer ABIN210984

Dieses Anti-p21-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von p21 in WB und IP. Geeignet für Human und Maus.

Kurzübersicht für p21 Antikörper (ABIN210984)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p21 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-p21 Antikörper

Spezifität: Recognizes WAF1, p21,~21kD. Species cross-reactivity: mouse.

Aufreinigung: Protein G purified

Immunogen: Recombinant full-length WAF1, p21 (Wild-type p53-activated Fragment, Cip1) with a histidine tag.

Type of Immunogen: Recombinant protein

Isotyp: IgG1, IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB

Usage: Suitable for use in Immunoprecipitation, Western Blot. Immunoprecipitation: 4 μg immunoprecipitates Cip1 from 500 μg of HeLa nuclear extract. Western Blot Analysis: 0.5-2 μg/mL detects WAF1, p21 in 20 μg of HeLa nuclear extract. HeLa nuclear extract was resolved by SDS PAGE, transferred to nitrocellulose, and probed with anti-WAF1, p21 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: HeLa nuclear extract. Use 20 μg per lane for minigels.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.5 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Andere Bezeichnung: CDKN1A / WAF1 / p21

Hintergrund: Name/Gene ID: CDKN1A

Synonyms: CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21

Gen-ID: 1026

UniProt: P38936

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagie