NF-kB p65 Antikörper (AA 297-308)
Kurzübersicht für NF-kB p65 Antikörper (AA 297-308) (ABIN209104)
Target
Alle NF-kB p65 (NFkBP65) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 297-308
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Spezifität
- Recognizes the nuclear localization sequence (NLS) NLS of human p65. The epitope overlaps the NLS of the p65 subunit of the NFkB heterodimer.
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Homologie
- Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Woolly monkey, Mouse, Rat, Hamster, Elephant, Panda, Porcine, Bovine, Dog, Bat, Horse (100%) Paramecium (83%).
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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A synthetic peptide, RIEEKRKRTYET, corresponding to the NLS of the human NFkB p65 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Woolly monkey, Mouse, Rat, Hamster, Elephant, Panda, Porcine, Bovine, Dog, Bat, Horse (100%), Paramecium (83%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
Isotyp
- IgG
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Applikationshinweise
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Approved: ELISA (1:100 - 1:1000), IF (1:200), IHC (1:100), WB (1:1000)
Usage: Suitable for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and Supershift assays. Western Blot: 1:1000. Immunohistochemistry: 1:100. Immunofluorescence microscopy: 1:100. Super shift: 0.5-1 μL/assay. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- PBS, pH 7.2, 0.1 % sodium azide, 40 % glycerol.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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Andere Bezeichnung
- RELA / NFKB p65
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Hintergrund
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Name/Gene ID: RELA
Synonyms: RELA, NF-kappa-B p65delta3, NFKB3, p65, Transcription factor p65 -
Gen-ID
- 5970
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UniProt
- Q04206
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Pathways
- NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine
Target
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