NF-kB p65 Antikörper

Produktnummer ABIN209098

Dieser Maus Monoklonal Antikörper detektiert spezifisch NF-kB p65 in WB, IHC, ELISA, IF, IHC (p), FACS, ICC, IHC (fro) und GS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für NF-kB p65 Antikörper (ABIN209098)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NF-kB p65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Gel Shift (GS)

Produktdetails anti-NF-kB p65 Antikörper

Spezifität: Recognizes an epitope overlapping the nuclear location signal (NLS) of the p65 subunit of the NFkB heterodimer. Thus it selectively binds to the activated form of NFkB.

Aufreinigung: Protein A purified

Immunogen: Peptide corresponding to human p65 coupled to BSA.

Type of Immunogen: Synthetic peptide

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, GS (1 - 10 μg/mL), ICC, IF, IHC, IHC-Fr (5 - 10 μg/mL), IHC-P (5 - 10 μg/mL), WB (5 - 10 μg/mL)

Usage: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Gel Supershift, Electrophoretic mobility supershift assays (EMSA), Immunohistochemistry and Immunocytochemistry. Immunofluorescence: 1-10 μg/mL. Flow cytometry: fixed cells only (acetone fixed cells). Western Blot: 5-10 μg/mL (ECL). Immunohistochemistry (Frozen, paraffin): 5-10 μg/mL, APAAP. HIER required Citrate buffer,  pH 6.0. Supershift assay: 0.5-1 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.6, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: RELA / NFKB p65

Hintergrund: Name/Gene ID: RELA

Synonyms: RELA, NF-kappa-B p65delta3, NFKB3, p65, Transcription factor p65

Gen-ID: 5970

UniProt: Q04206

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine