BRAF Antikörper (AA 754-765)
Kurzübersicht für BRAF Antikörper (AA 754-765) (ABIN205677)
Target
Alle BRAF Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
-
-
Bindungsspezifität
- AA 754-765
-
Spezifität
- Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species cross-reactivity: Rat and mouse.
-
Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%) Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%) Bat (83%).
-
Aufreinigung
- Protein A purified
-
Immunogen
-
Synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-terminal cysteine added for conjugation to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%), Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%), Bat (83%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
Isotyp
- IgG
-
-
-
-
Applikationshinweise
-
Approved: WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 μg/mL detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 μg. -
Kommentare
-
Target Species of Antibody: Human
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Konzentration
- Lot specific
-
Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide 30 % glycerol.
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handhabung
- Avoid repeat freeze-thaw cycles.
-
Lagerung
- 4 °C,-20 °C
-
Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
-
Andere Bezeichnung
- BRAF / B-Raf
-
Hintergrund
-
Name/Gene ID: BRAF
Subfamily: Raf
Family: Protein Kinase
Synonyms: BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1 -
Gen-ID
- 673
-
UniProt
- P15056
-
Pathways
- MAPK Signalweg, RTK Signalweg, Neurotrophin Signalübertragung, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagie
Target
-