TNF alpha Antikörper

Produktnummer ABIN199734

Der Maus Monoklonal anti-TNF alpha Antikörper (ABIN199734) detektiert spezifisch TNF alpha in WB, ELISA, ICC und Neut. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TNF alpha Antikörper (ABIN199734)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Neutralization (Neut)

Produktdetails anti-TNF alpha Antikörper

Spezifität: Recognizes recombinant human TNF-a. Cross-reactivity: rhTNF-b, rmTNF-a, rrTNF-a, rpTNF-a, rhTNF RI, rhTNF RII, rmTNF RI and rmTNF RII: >0.05 % .

Aufreinigung: Protein G purified

Immunogen: RhTNR-a expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (4 μg/mL), ICC, Neut, WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Neutralization. ELISA: 4 μg/mL as a capture antibody. Use 100 μL/well. Western Blot: ~1-2 μg/mL. The detection limit for rhTNFa is 5 ng/lane under non-reducing and reducing conditions using colorimetric detection. 5-50-fold increased sensitivity can be obtained using ECL. Neutralization of Bioactivity: 0.04-0.08 μg/mL typically neutralizes 50 % of the bioactivity of 0.25 ng/mL of rhTNF-a.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF Alpha

Hintergrund: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gen-ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome