Ephrin B2 Antikörper (Tyr316)

Produktnummer ABIN197416

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Ephrin B2 in WB. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Ephrin B2 Antikörper (Tyr316) (ABIN197416)

Target: Ephrin B2 (EFNB2)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ephrin B2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Ephrin B2 Antikörper

Bindungsspezifität: Tyr316

Spezifität: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Ephrin-B2 antibody AP02707PU detects endogenous levels of total Ephrin-B2 protein.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Ephrin-B2 around the phosphorylation site of tyrosine 316 (P-V-Yp-I-V).

Anwendungsinformationen

Applikationshinweise: Suitable for use in in Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu Ephrin B2

Target: Ephrin B2 (EFNB2)

Andere Bezeichnung: Ephrin-B2

Hintergrund: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP)and also interacts with PRKCABP and GRIP1. This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. Ephrin-B2 also binds EphA4, EphB1, EphB2, EphB3 and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.Synonyms: EFNB2, EPH-related receptor tyrosine kinase ligand 5, EPLG5, HTK ligand, HTK-L, HTKL, LERK-5, LERK5

Gen-ID: 1948

NCBI Accession: NP_004084

UniProt: P52799

Pathways: RTK Signalweg, Regulation of Muscle Cell Differentiation