AKT2 Antikörper (Ser474)

Produktnummer ABIN197358

Der Kaninchen Polyklonal anti-AKT2 Antikörper (ABIN197358) detektiert spezifisch AKT2 in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für AKT2 Antikörper (Ser474) (ABIN197358)

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AKT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-AKT2 Antikörper

Bindungsspezifität: Ser474

Spezifität: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Akt2 antibody AP02678PU detects endogenous levels of total Akt2 protein.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-Sp-Y-S).

Anwendungsinformationen

Applikationshinweise: Suitable for use in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Andere Bezeichnung: AKT2 / PKB beta

Hintergrund: The serine/threonine kinase Akt (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3. AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase

Gen-ID: 208

NCBI Accession: NP_001617

UniProt: P31751

Pathways: PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, TLR Signalweg, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling