ATF2 Antikörper (Ser44, Ser62)

Produktnummer ABIN197144

Produktdetails anti-ATF2 Antikörper

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Bindungsspezifität: Ser44, Ser62

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Spezifität: ATF-2 antibody detects endogenous levels of total ATF-2 protein.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic non-phosphopeptide derived from human ATF-2 around the phosphorylation site of serine 62 or 44 (N-D-SP-V-I).

Anwendungsinformationen

Applikationshinweise: Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu ATF2

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Andere Bezeichnung: ATF2 (ATF2 Produkte)

Synonyme: CRE-BP1 antikoerper, CREB2 antikoerper, HB16 antikoerper, TREB7 antikoerper, CG12850 antikoerper, CG3749 antikoerper, dATF-2 antikoerper, DmelCG30420 antikoerper, CG30420 antikoerper, CHN1 antikoerper, Atf-2 antikoerper, CRE-BP antikoerper, Creb2 antikoerper, D130078H02Rik antikoerper, D18875 antikoerper, Tg(Gzma-Klra1)7Wum antikoerper, mXBP antikoerper, atf2-A antikoerper, atf2l antikoerper, activating transcription factor 2 antikoerper, Activating transcription factor-2 antikoerper, activating transcription factor 2 S homeolog antikoerper, ATF2 antikoerper, Atf-2 antikoerper, Atf2 antikoerper, atf2.S antikoerper, atf2 antikoerper

Hintergrund: ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.Synonyms: ATF-2, Activating transcription factor 2, CRE-BP1, CREB-2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, cAMP-dependent transcription factor ATF-2

Gen-ID: 1386

NCBI Accession: NP_001871

UniProt: P15336

Pathways: MAPK Signalweg, RTK Signalweg, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades