MAP2K3 Antikörper (pSer189)

Produktnummer ABIN196862

Der Kaninchen Polyklonal Anti-MAP2K3-Antikörper wurde für WB, IF und IHC (p) validiert. Er ist geeignet, MAP2K3 in Proben von Human und Ratte zu detektieren.

Kurzübersicht für MAP2K3 Antikörper (pSer189) (ABIN196862)

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP2K3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-MAP2K3 Antikörper

Bindungsspezifität: pSer189

Spezifität: MKK3 (phospho-Ser189) antibody detects endogenous levels of MKK3 only when phosphorylated at serine189.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: Synthetic phosphopeptide derived from human MKK3 around the phosphorylation site of serine 189 (V-D-SP-V-A).

Anwendungsinformationen

Applikationshinweise: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu MAP2K3

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Andere Bezeichnung: MAP2K3

Hintergrund: MEK3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MEK3, MKK3

Gen-ID: 5606

NCBI Accession: NP_002747

UniProt: P46734

Pathways: MAPK Signalweg, TLR Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling Events mediated by VEGFR1 and VEGFR2