BRAF Antikörper

Produktnummer ABIN1881118

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch BRAF in WB und FACS. Er zeigt eine Reaktivität gegenüber Human und wurde in 6+ Publikationen erwähnt.

Kurzübersicht für BRAF Antikörper (ABIN1881118)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRAF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: RB22681

Produktdetails anti-BRAF Antikörper

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This BRAF antibody is generated from rabbits immunized with BRAF recombinant protein.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. FC: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-BRAF Antikörper (ABIN1881118)

Weisbart, Chan, Heinze, Mory, Nishimura, Colburn: "BRAF drives synovial fibroblast transformation in rheumatoid arthritis." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 34299-303, (2010) (PubMed).

Hingorani, Jacobetz, Robertson, Herlyn, Tuveson: "Suppression of BRAF(V599E) in human melanoma abrogates transformation." in: Cancer research, Vol. 63, Issue 17, pp. 5198-202, (2003) (PubMed).

Lee, Yoo, Soung, Kim, Park, Kim, Lee, Park, Cho, Kim, Ko, Kim, Nam, Lee, Lee: "BRAF mutations in non-Hodgkin's lymphoma." in: British journal of cancer, Vol. 89, Issue 10, pp. 1958-60, (2003) (PubMed).

Davies, Bignell, Cox, Stephens, Edkins, Clegg, Teague, Woffendin, Garnett, Bottomley, Davis, Dicks, Ewing, Floyd, Gray, Hall, Hawes, Hughes, Kosmidou, Menzies, Mould, Parker, Stevens, Watt, Hooper et al.: "Mutations of the BRAF gene in human cancer. ..." in: Nature, Vol. 417, Issue 6892, pp. 949-54, (2002) (PubMed).

Naoki, Chen, Richards, Sugarbaker, Meyerson: "Missense mutations of the BRAF gene in human lung adenocarcinoma." in: Cancer research, Vol. 62, Issue 23, pp. 7001-3, (2002) (PubMed).

Stephens, Sithanandam, Copeland, Kaplan, Rapp, Morrison: "95-kilodalton B-Raf serine/threonine kinase: identification of the protein and its major autophosphorylation site." in: Molecular and cellular biology, Vol. 12, Issue 9, pp. 3733-42, (1992) (PubMed).

Details zu BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Andere Bezeichnung: BRAF

Hintergrund: BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

Molekulargewicht: 84437

NCBI Accession: NP_004324

UniProt: P15056

Pathways: MAPK Signalweg, RTK Signalweg, Neurotrophin Signalübertragung, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagie