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CD14 Antikörper (Internal Region)

Name: CD14 Antikörper (Internal Region)
Brand: antibodies-online
SKU: ABIN185563
Price: 575.06 EUR
Availability: InStock

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch CD14 in FACS, WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

(ABIN185563) Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0. (CD14 Antikörper (Internal Region))

FACS

(ABIN185563) (2 μg/mL) staining of Human Lymph node (A) + peptide (B) and Tonsil (C) + peptide (D) lysate (35 μg protein in RIPA buffer) Detected by chemiluminescence. (CD14 Antikörper (Internal Region))

ABIN185563 (1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). (CD14 Antikörper (Internal Region))

ABIN185563 (1µg/ml) staining of PBM lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. (CD14 Antikörper (Internal Region))

ABIN185563 (4µg/ml) staining of paraffin embedded Human Spleen. (CD14 Antikörper (Internal Region))

IHC

5 Abbildungen

Produktnummer ABIN185563

Kurzübersicht für CD14 Antikörper (Internal Region) (ABIN185563)

Target

Alle CD14 Antikörper anzeigen

CD14

Reaktivität

Human

Wirt

Ziege

Klonalität

Polyklonal

Konjugat

Dieser CD14 Antikörper ist unkonjugiert

Applikation

Flow Cytometry (FACS), Western Blotting (WB), ELISA

Güteklasse

Verified

Discover our top product CD14 Primärantikörper

Bindungsspezifität
59

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Internal Region

Verwendungszweck

CD14

Sequenz

KRVDADADPR QYAD

Kreuzreaktivität

Human

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen

Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.

Isotyp

IgG
anti-CD14 (CD14) (AA 54-83), (N-Term) antibody
CD14 Reaktivität: Human FACS, WB, IF, IHC (p) Wirt: Kaninchen Polyclonal RB14105 unconjugated

anti-CD14 (CD14) (AA 20-214) antibody
CD14 Reaktivität: Human IHC, ELISA, ICC Wirt: Maus Monoclonal 1H5D8 unconjugated

anti-CD14 (CD14) (AA 20-214) antibody
CD14 Reaktivität: Human, Maus FACS, WB, IHC, ELISA Wirt: Maus Monoclonal 4B4F12 unconjugated

anti-CD14 (CD14) (AA 20-345) antibody
CD14 Reaktivität: Human FACS, WB, IHC, ELISA, IF Wirt: Maus Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) antibody
CD14 Reaktivität: Human IHC, ELISA Wirt: Maus Monoclonal 1A3B6 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 Reaktivität: Human FACS, WB, IHC, ELISA Wirt: Maus Monoclonal 1D1F8 unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 Reaktivität: Human FACS, WB, IHC, ISt, StM Wirt: Maus Monoclonal LPSR-2385 unconjugated

anti-CD14 (CD14) antibody
CD14 Reaktivität: Human, Non-Human Primate FACS, WB, ELISA, IP, Func Wirt: Maus Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody
CD14 Reaktivität: Human, Non-Human Primate FACS, WB, ELISA, IP Wirt: Maus Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody (Biotin)
CD14 Reaktivität: Human, Non-Human Primate FACS, WB, ELISA, IP Wirt: Maus Monoclonal MEM-18 Biotin

Applikationshinweise

Western Blot: Approx 55 kDa band observed in Human Lymph node and Tonsil lysates (calculated MW of 40.1 kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form, and was successfully blocked by incubation with the im
Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

CD14

Andere Bezeichnung

CD14

Hintergrund

CD14, CD14 antigen , HGNC:1628

Gen-ID

929

NCBI Accession

NP_000582

Pathways

TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades