PYCARD Antikörper (C-Term)

Produktnummer ABIN184674

Dieses Anti-PYCARD-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von PYCARD in WB, ELISA und IHC. Geeignet für Human.

Kurzübersicht für PYCARD Antikörper (C-Term) (ABIN184674)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PYCARD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-PYCARD Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: ASC / TMS1

Sequenz: RESQSYLVED LERS

Spezifität: This antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1).

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RESQSYLVEDLERS, from the C Terminus of the protein sequence according to NP_037390.2, NP_660183.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Liver shows staining of sinusoidal lining cells. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 19 kDa band observed in lysates of cell line HeLa (calculated MW of 20.0 kDa according to NP_660183.1). Recommended concentration: 0.2-0.6 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Andere Bezeichnung: PYCARD

Hintergrund: PYCARD, ASC, TMS1, PYD and CARD domain containing, CARD5, MGC10332, TMS, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1, TMS-1

Gen-ID: 29108

NCBI Accession: NP_037390, NP_660183

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome