SMAD4 Antikörper (C-Term)

Produktnummer ABIN184619

Der Ziege Polyklonal Anti-SMAD4-Antikörper wurde für ELISA und IP validiert. Er ist geeignet, SMAD4 in Proben von Human zu detektieren.

Kurzübersicht für SMAD4 Antikörper (C-Term) (ABIN184619)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SMAD4 Antikörper ist unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP)

Güteklasse: Verified

Produktdetails anti-SMAD4 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: SMAD4 / MADH4

Sequenz: HTMPIADPQP LD

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HTMPIADPQPLD, from the C Terminus of the protein sequence according to NP_005350.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary experiments gave a band at approx 75 kDa in lysates of cell line NIH3T3. Please note that currently we cannot find an explanation in the literature

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Andere Bezeichnung: SMAD4

Hintergrund: MADH4, SMAD4, JIP, DPC4, MAD, mothers against decapentaplegic homolog 4 (Drosophila), Mothers against decapentaplegic, Drosophila, homolog of, 4, MAD (mothers against decapentaplegic, Drosophila) homolog 4, SMAD family member 4, OTTHUMP00000163548, SMAD,

Gen-ID: 4089, 50554

NCBI Accession: NP_005350

Pathways: Zellzyklus, Chromatin Binding, Autophagie