BRAF Antikörper

Produktnummer ABIN1685167

Der Kaninchen Polyklonal anti-BRAF Antikörper wird verwendet zum Nachweis von BRAF in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA und IHC.

Kurzübersicht für BRAF Antikörper (ABIN1685167)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRAF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-BRAF Antikörper

Aufreinigung: Purified

Sterilität: Sterile filtered

Immunogen: Polyclonal BRAF antibody was produced from sera of rabbits immunized with synthetic protein containing BRAF antigenic determinants.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: Use at a concentration range of 1-2 µg/mL.
Western blot: A suitable range of concentrations of this antibody for WB detection is 10 µg/mL.
IHC: A suitable range of concentrations of this antibody for IHC is 10 µg/mL. Immunohistochemical analysis of BRAF expression in paraffin embedded formalin fixed Rat brain tissue. ABIN1685167 was used as primary antibody, dilution 1:200.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

Buffer: PBS solution at a concentration of 1 mg/mL.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Recombinant human CD117, as supplied, can be stored in working aliquots at - 8 °C for one month, or at -20 °C to -70 °C for twelve months.

Haltbarkeit: 12 months

Details zu BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Andere Bezeichnung: BRAF

Hintergrund: The c-Kit receptor consists of an extracellular portion with five immunoglobulin-like regions and an intracellular tyrosine kinase domain. The first three immunoglobulin-like domains form a binding site for its ligand, stem cell factor (SCF), the fourth is involved in c-Kit receptor dimerization, and the function of the fifth is, as yet, unknown. Binding of SCF induces homodimerization of the c-Kit receptor, followed by an increase in receptor autophosphorylation activity and recruitment of the c-Kit signaling complex.

UniProt: P15056

Pathways: MAPK Signalweg, RTK Signalweg, Neurotrophin Signalübertragung, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagie