PKC beta Antikörper (Internal Region)

Produktnummer ABIN1590084

Dieses Anti-PKC beta-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von PKC beta in WB, ELISA und IHC. Geeignet für Human und Ratte.

Kurzübersicht für PKC beta Antikörper (Internal Region) (ABIN1590084)

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PKC beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-PKC beta Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: PRKCB

Sequenz: DKEFTRQPVE LT

Spezifität: This antibody is expected to recognize isoform 1 (NP_997700.1) only.

Kreuzreaktivität: Hund, Human, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9 kDa according to NP_997700.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PKC beta

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Andere Bezeichnung: PRKCB

Hintergrund: PRKCB, protein kinase C, beta, MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2, OTTHUMP00000162377, OTTHUMP00000162378, PKC-B, protein kinase C beta type, protein kinase C, beta 1 polypeptide

Gen-ID: 5579

NCBI Accession: NP_997700

Pathways: WNT Signalweg, T-Zell Rezeptor Signalweg, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling