Patched 1 Antikörper (AA 266-280)

Produktnummer ABIN1590007

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Patched 1 in WB, ELISA, IHC und IF. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Patched 1 Antikörper (AA 266-280) (ABIN1590007)

Target: Patched 1 (PTCH1)

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Patched 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-Patched 1 Antikörper

Bindungsspezifität: AA 266-280

Verwendungszweck: PTCH1 (aa266-280)

Sequenz: EELKKINYQV DSWEE

Spezifität: This antibody is expected to recognize all reported isoforms (NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077073.1). Reported variants represent identical protein: NP_001077075.1, NP_001077074.1, NP_001077073.1, NP_001077076.1

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EELKKINYQVDSWEE, from the C or N Terminus of the protein sequence according to NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077073.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 8 μg/mL.

Western Blot: Approx 140 kDa band observed in Mouse fetal Kidney lysates (calculated MW of 159 kDa according to NP_032983.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Patched 1

Target: Patched 1 (PTCH1)

Andere Bezeichnung: PTCH1

Hintergrund: PTCH1, patched 1, BCNS, FLJ26746, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched homolog 1, protein patched homolog 1

Gen-ID: 5727, 19206, 89830

NCBI Accession: NP_000255, NP_001077072, NP_001077071, NP_001077073

Pathways: Hedgehog Signalweg, Carbohydrate Homeostasis, Tube Formation