Caspase 8 Antikörper (AA 188-280)

Produktnummer ABIN1386778

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Caspase 8 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für Caspase 8 Antikörper (AA 188-280) (ABIN1386778)

Target: Caspase 8 (CASP8)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Caspase 8 Antikörper

Bindungsspezifität: AA 188-280

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Caspase-8 subunit p18

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Caspase 8 Antikörper (ABIN1386778)

Shan, Fan: "Cytotoxicity of carteolol to human corneal epithelial cells by inducing apoptosis via triggering the Bcl-2 family protein-mediated mitochondrial pro-apoptotic pathway." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 35, pp. 36-42, (2016) (PubMed).

Zhao, Qiu, Tian, Fan: "The cytotoxic and pro-apoptotic effects of phenylephrine on corneal stromal cells via a mitochondrion-dependent pathway both in vitro and in vivo." in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, Vol. 68, Issue 7, pp. 409-17, (2016) (PubMed).

Bozok Cetintas, Acikgoz, Yigitturk, Demir, Oktem, Tezcanli Kaymaz, Oltulu, Aktug: "Effects of flavopiridol on critical regulation pathways of CD133high/CD44high lung cancer stem cells." in: Medicine, Vol. 95, Issue 43, pp. e5150, (2016) (PubMed).

Meneses, Morales, Abrigo, Simon, Brandan, Cabello-Verrugio: "The angiotensin-(1-7)/Mas axis reduces myonuclear apoptosis during recovery from angiotensin II-induced skeletal muscle atrophy in mice." in: Pflügers Archiv : European journal of physiology, (2014) (PubMed).

Details zu Caspase 8

Target: Caspase 8 (CASP8)

Andere Bezeichnung: Caspase-8

Hintergrund:

Synonyms: CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, Caspase-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, CASP8

Background: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Gen-ID: 841

UniProt: Q14790

Pathways: Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades