LY9 Antikörper (APC)

Produktnummer ABIN1302635

Der Maus Monoklonal Anti-LY9-Antikörper wurde für FACS validiert. Er ist geeignet, LY9 in Proben von Human zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für LY9 Antikörper (APC) (ABIN1302635)

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LY9 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Klon: HLy9-25

Produktdetails anti-LY9 Antikörper (APC)

Verwendungszweck: Anti-Hu CD229 APC

Spezifität: The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: CD299-transfected 300.19 pre-B cell line

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-LY9 Antikörper (APC) (ABIN1302635)

Romero, Zapater, Calvo, Kalko, de la Fuente, Tovar, Ockeloen, Pizcueta, Engel: "CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 7033-42, (2005) (PubMed).

de la Fuente, Tovar, Villamor, Zapater, Pizcueta, Campo, Bosch, Engel: "Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9." in: Blood, Vol. 97, Issue 11, pp. 3513-20, (2001) (PubMed).

Details zu LY9

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Andere Bezeichnung: CD229

Hintergrund: Lymphocyte antigen 9,CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.,SLAMF3, LY9

Gen-ID: 4063

UniProt: Q9HBG7

Pathways: BCR Signaling