Caspase 8 Antikörper (active, Cleavage Site)

Produktnummer ABIN127031

Der Kaninchen Polyklonal Anti-Caspase 8-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, Caspase 8 in Proben von Human zu detektieren.

Kurzübersicht für Caspase 8 Antikörper (active, Cleavage Site) (ABIN127031)

Target: Caspase 8 (CASP8)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Caspase 8 Antikörper

Bindungsspezifität: active, Cleavage Site

Spezifität: The affinity purified antibody mainly detects the 26 kDa fully cleaved fragment and the 35-43 kDa intermediate of caspase-8 in apoptotic samples. Other species have not been tested.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide surrounding the cleavage site of human Caspase-8

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5-4 μg/mL. Immunohistochemistry: 10-20 mg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Details zu Caspase 8

Target: Caspase 8 (CASP8)

Andere Bezeichnung: Caspase-8

Hintergrund: Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MCH5, MORT1-associated CED-3 homolog

Gen-ID: 841

NCBI Accession: NP_001073593

UniProt: Q14790

Pathways: Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades