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Caspase 9 Antikörper (Asp330, N-Term)

Der Kaninchen Polyklonal Anti-Caspase 9-Antikörper wurde für WB, IF, IP und IHC (p) validiert. Er ist geeignet, Caspase 9 in Proben von Human zu detektieren.
Produktnummer ABIN127028

Kurzübersicht für Caspase 9 Antikörper (Asp330, N-Term) (ABIN127028)

Target

Alle Caspase 9 (CASP9) Antikörper anzeigen
Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reaktivität

  • 238
  • 139
  • 110
  • 17
  • 17
  • 16
  • 4
Human

Wirt

  • 227
  • 29
Kaninchen

Klonalität

  • 208
  • 48
Polyklonal

Konjugat

  • 143
  • 11
  • 10
  • 7
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 1
Dieser Caspase 9 Antikörper ist unkonjugiert

Applikation

  • 225
  • 85
  • 76
  • 71
  • 67
  • 67
  • 43
  • 40
  • 38
  • 32
  • 16
  • 5
  • 3
  • 3
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 28
    • 16
    • 16
    • 16
    • 16
    • 11
    • 9
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Asp330, N-Term

    Spezifität

    The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. The anti-active caspase-9 antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspases.

    Aufreinigung

    Affinity chromatography

    Immunogen

    Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human Caspase-9
  • Applikationshinweise

    Western Blot: 0.5-4 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry /Immunocytochemistry: 10-20 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    0.2 mg/mL

    Buffer

    PBS containing 50 % glycerol, 0.5 % BSA, and 0.02 % thimerosal

    Konservierungsmittel

    Thimerosal (Merthiolate)

    Vorsichtsmaßnahmen

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.
  • Target

    Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

    Andere Bezeichnung

    Caspase-9

    Hintergrund

    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved Caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6

    Gen-ID

    842

    NCBI Accession

    NP_001220

    UniProt

    P55211

    Pathways

    MAPK Signalweg, RTK Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Endopeptidase Activity
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