MAP2K3 Antikörper

Produktnummer ABIN126840

Der Maus Monoklonal anti-MAP2K3 Antikörper (Klon 5F7) (ABIN126840) detektiert spezifisch MAP2K3 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Hund.

Kurzübersicht für MAP2K3 Antikörper (ABIN126840)

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Reaktivität: Human, Maus, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAP2K3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: 5F7

Produktdetails anti-MAP2K3 Antikörper

Spezifität: This antibody specifically recognizes the N-terminus of MKK3.

Produktmerkmale: Synonyms: MAPKK 3, MEK3, MKK3, MAP kinase kinase 3, Dual specificity mitogen-activated proteinkinase kinase 3

Aufreinigung: Size exclusion chromatography

Bestandteile: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated A431 cells. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu MAP2K3

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Andere Bezeichnung: MAP2K3

Hintergrund: MKK3 activates p38 MAP kinase by phosphorylating a Thr and Tyr residue in the activation loop. MKK3 does not phosphorylate and activate the other major MAP kinases, MAPK1/2 (erk1/2) or SAPK/JNK. Cellular stress and inflammatory cytokines activate MKK3 and lead to phosphorylation of Ser 189 and Thr 193.Synonyms: Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MEK3, MKK3

Gen-ID: 5606

UniProt: P46734

Pathways: MAPK Signalweg, TLR Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling Events mediated by VEGFR1 and VEGFR2