ERK1 Antikörper (pThr-Glu-pTyr)

Produktnummer ABIN126831

Der Maus Monoklonal anti-ERK1 Antikörper (Klon 12D4) (ABIN126831) detektiert spezifisch ERK1 in WB, IF, IP, IHC (fro) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Hund.

Kurzübersicht für ERK1 Antikörper (pThr-Glu-pTyr) (ABIN126831)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ERK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 12D4

Produktdetails anti-ERK1 Antikörper

Bindungsspezifität: pThr-Glu-pTyr

Spezifität: This antibody specifically interacts with the pThr - Glu - pTyr motif of activated MAP kinases 1 and 2 (erk1/2). The antibody requires phosphorylation both at the threonine and the tyrosine site and does not interact with the non-phosphorylated form of the protein. Mab MAPK-12D4 shows no crossreaction with activated SAP kinases 1 or 2.

Produktmerkmale: Synonyms: ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK, Extracellular signal regulated kinase 1/2,Insulin stimulated MAP2 kinase, Microtubule associated protein 2 kinase, Mitogenactivated protein kinase 1/2/3

Aufreinigung: Size exclusion chromatography

Bestandteile: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from pervanadate-treated HepG2 cells. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 g per 10^6 pervanadate-treated A431 or HepG2 cells. Immunocytochemistry: 1 - 10 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Andere Bezeichnung: MAPK3 / ERK1

Hintergrund: Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK

Gen-ID: 5595

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteine