JNK Antikörper

Produktnummer ABIN126827

Der Maus Monoklonal Anti-JNK Antikörper wurde für WB, IP, IHC (fro) und EIA validiert. Er ist geeignet, JNK in Proben von Human und Maus zu detektieren.

Kurzübersicht für JNK Antikörper (ABIN126827)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser JNK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 9H8

Produktdetails anti-JNK Antikörper

Spezifität: This antibody specifically interacts with the Thr - Gly/Pro - pTyr motif of activated, monophosphorylated SAPK1/2 kinases. The antibody accepts the dually phosphorylated site as well (pThr - Gly/Pro - pTyr) and does not interact with the non-phosphorylated form of the protein. Mab SAPK1/2-9H8 shows no crossreaction with activated MAP Kinases 1 or 2.

Produktmerkmale: Synonyms: Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8,Stress-activated protein kinase JNK1

Aufreinigung: Size exclusion chromatography

Bestandteile: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from sorbit-treated A431 cells. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 g per 10^6 pervanadate-treated A431 cells. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.1 % Na-azide / PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Andere Bezeichnung: MAPK8 / JNK1

Hintergrund: Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines.Synonyms: JNK-1, JNK-46, Mitogen-activated protein kinase 8, PRKM8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1

Gen-ID: 5599

UniProt: P45983

Pathways: MAPK Signalweg, WNT Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteine