JNK Antikörper (N-Term) (Biotin)

Produktnummer ABIN126825

Der Biotin-konjugierte Maus Monoklonal anti-JNK Antikörper (Klon 5D10) (ABIN126825) detektiert spezifisch JNK in WB, IHC (fro) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Hund.

Kurzübersicht für JNK Antikörper (N-Term) (Biotin) (ABIN126825)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser JNK Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 5D10

Produktdetails anti-JNK Antikörper (Biotin)

Bindungsspezifität: N-Term

Spezifität: This antibody specifically interacts with the N-terminus of SAPK1γ/jnk1 kinases.

Produktmerkmale: Synonyms: Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8,Stress-activated protein kinase JNK1

Aufreinigung: Size exclusion chromatography

Bestandteile: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.05 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS/0.09 % Na-Azide/PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-80 °C

Informationen zur Lagerung: Store the antibody (aliquote in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Andere Bezeichnung: MAPK8 / JNK1

Hintergrund: Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1α/jnk2, SAPK1β/jnk3, SAPK1γ/jnk1.Synonyms: JNK-1, JNK-46, Mitogen-activated protein kinase 8, PRKM8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1

Gen-ID: 5599

UniProt: P45983

Pathways: MAPK Signalweg, WNT Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteine