CD14 Antikörper

Produktnummer ABIN126002

Der Maus Monoklonal anti-CD14 Antikörper wird verwendet zum Nachweis von CD14 in Proben von Human und Primate. Er wurde validiert für FACS, WB, IP, Func und EIA.

Kurzübersicht für CD14 Antikörper (ABIN126002)

Target: CD14

Reaktivität: Human, Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Enzyme Immunoassay (EIA)

Klon: MEM-18

Produktdetails anti-CD14 Antikörper

Aufreinigung: Protein A Chromatography

Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry. Immunoprecipitation. Western blotting (non-denaturing conditions). ELISA: antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with clone B-A8 (ABIN120555). Functional application: completely blocks binding of fluorescein (FITC) labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Azide Free Phosphate Buffered Saline (PBS), pH ~7.4, 0.2 μm filter sterilized. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.

Konservierungsmittel: Azide free

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor, it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades