LAT2 Antikörper (Linker For Activation of T Cells Family, Member 2) (AA 91-244)

Details for Product anti-LAT2 Antibody No. ABIN120727
Antigen
  • LAB
  • NTAL
  • WBSCR15
  • WBSCR5
  • WSCR5
  • LAT2
  • MGC139435
  • Ntal
  • Wbscr5
  • AW125574
  • Wbscr15
  • linker for activation of T-cells family member 2
  • linker for activation of T cells family, member 2
  • LAT2
  • Lat2
Epitop
AA 91-244
16
15
11
10
10
10
9
8
8
6
5
5
5
4
2
2
2
2
2
1
1
1
1
1
Reaktivität
Human, Maus
122
42
31
3
3
3
1
1
1
1
1
Wirt
Maus
108
28
14
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser LAT2 Antikörper ist unkonjugiert
8
8
6
3
3
3
2
2
2
2
2
2
Applikation
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
131
43
31
29
16
12
12
6
4
3
3
2
1
Optionen
Immunogen Recombinant cytoplasmic domain (aa 91-244) of human NTAL
Klon NAP-07
Isotyp IgG1
Spezifität The antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
Reinigung Protein A affinity chromatography
Reinheit > 95 % (by SDS-PAGE)
Plasmids, Primers & others Plasmide, Primers & weitere LAT2 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung LAT2 / WBSCR15 (LAT2 Antibody Abstract)
Hintergrund NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein
Gen-ID 7462
UniProt Q9GZY6
Pathways Fc-epsilon Rezeptor Signalübertragung
Applikations-hinweise Immunoprecipitation. Western Blot: Recommended dilution: 2 mg/mL, incubation 60 min Positive control: RAMOS human lymphoma cell line Negative control: HeLa human cervix carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12 % separating SDS-PAGE gel. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Haltbarkeit 12 months
Produkt verwendet in: Nørsett, Laegreid, Langaas, Wörlund, Fossmark, Waldum, Sandvik: "Molecular characterization of rat gastric mucosal response to potent acid inhibition." in: Physiological genomics, Vol. 22, Issue 1, pp. 24-32, 2007 (PubMed).

Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, 2004 (PubMed).

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, 2004 (PubMed).

Allgemeine Veröffentlichungen Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

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