TNF alpha Antikörper

Produktnummer ABIN120182

Produktdetails anti-TNF alpha Antikörper

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Spezifität: This antibody recognises Bovine TNF alpha.

Keine Kreuzreaktivität: Schaf

Kreuzreaktivität (Details): Species reactivity (tested):Bovine.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Recombinant bovine TNF alpha.

Klon: CC327

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: This reagent may be used as a capture antibody in ELISA assays for bovine and sheep TNF alpha in combination with ABIN120216 as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-TNF alpha Antikörper (ABIN120182)

Studer, Collette, Whelan, Albrecht, Casselman, de Reijke, Knoenagel, Loidl, Isorna, Sundaram, Debois,: "Using PSA to Guide Timing of Androgen Deprivation in Patients with T0-4 N0-2 M0 Prostate Cancer not Suitable for Local Curative Treatment (EORTC 30891)." in: European urology, (2008) (PubMed).

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor (TNF-Alpha) (TNF alpha Produkte)

Hintergrund: Tumor Necrosis Factor alpha (TNF alpha) is a 17.5 kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes. The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

Gen-ID: 280943

NCBI Accession: NP_776391

UniProt: Q06599

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome