GZMB Antikörper

Produktnummer ABIN119563

Dieses Maus Monoklonal-Antikörper erkennt spezifisch GZMB in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für GZMB Antikörper (ABIN119563)

Target: GZMB (Granzyme B (GZMB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GZMB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: GB7

Produktdetails anti-GZMB Antikörper

Spezifität: This antibody recognises the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Recombinant Granzyme B. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Paraffin Sections (1/50-1/200): Requires antigen retrieval using heat treatment with sodium citrate buffer pH 6.0. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: TRIS buffered saline, pH 8.0, 0.01 % Thiomersal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GZMB

Target: GZMB (Granzyme B (GZMB))

Hintergrund: Granzyme A and Granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes, and they are stored within cytolytic granules and released by effector cells during degranulation. In activated CTLs, granzyme A and granzyme B are processed and activated by cathepsin C, and they then function to induce apoptosis by two distinct pathways. Granzyme B proteolytically cleaves and activates members of the caspase family of cysteine proteases, including caspase-3, caspase-6, caspase-7 and caspase-9. When cleaved, these caspases assemble into active holoenzymes that then mediate apoptosis through a defined proteolytic cascade involving nuclear lamins and PARP (poly ADP ribose polymerase). Granzyme A mediates the activation of apoptosis by inducing single-strand DNA breaks, membrane perturbation and nuclear condensations in an alternative pathway that is independent from caspase activation or the caspase proteolytic cascade.Synonyms: CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, Granzyme-2, Lymphocyte protease, SECT, T-cell serine protease 1-3E

Gen-ID: 3002

NCBI Accession: NP_004122

UniProt: P10144

Pathways: Apoptose, Caspase Kaskade in der Apoptose