GZMB Antikörper (Granzyme B)

Produktnummer ABIN1302451

Produktdetails anti-GZMB Antikörper

Target: GZMB

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GZMB Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Spezifität: The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed in activated Tc cells and NK cells.

Kreuzreaktivität (Details): Human, Non-Human Primates

Aufreinigung: Purified by protein-A affinity chromatography

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Human NK cell line YT-INDY-derived granzyme B

Klon: CLB-GB11

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

Referenzen für anti-GZMB Antikörper (ABIN1302451)

Rissoan, Duhen, Bridon, Bendriss-Vermare, Péronne, de Saint Vis, Brière, Bates: "Subtractive hybridization reveals the expression of immunoglobulin-like transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells." in: Blood, Vol. 100, Issue 9, pp. 3295-303, (2002) (PubMed).

Tak, Spaeny-Dekking, Kraan, Breedveld, Froelich, Hack: "The levels of soluble granzyme A and B are elevated in plasma and synovial fluid of patients with rheumatoid arthritis (RA)." in: Clinical and experimental immunology, Vol. 116, Issue 2, pp. 366-70, (1999) (PubMed).

Spaeny-Dekking, Hanna, Wolbink, Wever, Kummer, Kummer, Swaak, Middeldorp, Huisman, Froelich, Hack: "Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 7, pp. 3610-6, (1998) (PubMed).

Details zu GZMB

Target: GZMB

Andere Bezeichnung: Granzyme B (GZMB Produkte)

Synonyme: CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT, granzyme B, GZMB

Hintergrund: Granzyme B is a serine protease that is expressed in cytoplasmic granules of cytotoxic T lymphocytes and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Granzyme B plays a pivotal role in the induction of apoptosis in the target cells by activation of caspases. Moreover, granzyme B was reported to cleave directly alpha-tubulin, leading to perturbation of microtubule networks during the induced cell death.

Pathways: Apoptose, Caspase Kaskade in der Apoptose