TNF alpha Antikörper
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- Target Alle TNF alpha Antikörper anzeigen
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Reaktivität
- Maus
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Wirt
- Ratte
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Klonalität
- Monoklonal
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Konjugat
- Dieser TNF alpha Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Aufreinigung
- Affinity chromatography on Protein G
- Immunogen
- Recombinant murine TNF alpha expressed in E. coli.
- Klon
- MP6-XT22
- Isotyp
- IgG1
- Top Product
- Discover our top product TNF alpha Primärantikörper
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- Applikationshinweise
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Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA: 1 μg/mL - 10 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4, 0.09 % Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Andere Bezeichnung
- Tumor Necrosis Factor (TNF-Alpha) (TNF alpha Produkte)
- Hintergrund
- TNA alpha is a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immuno-regulatory role.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
- Gen-ID
- 10090
- UniProt
- P06804
- Pathways
- NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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