TNF alpha Antikörper

Produktnummer ABIN119358

Dieser Anti-TNF alpha Antikörper ist ein Ratte Monoklonal Antikörper zur Detektion von TNF alpha in FACS, IHC (p), Func, EIA und IHC (fro). Geeignet für Maus und Ratte.

Kurzübersicht für TNF alpha Antikörper (ABIN119358)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Maus, Ratte

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: MP6-XT22

Produktdetails anti-TNF alpha Antikörper

Aufreinigung: Affinity Chromatography on Protein G

Endotoxin-Niveau: less than 0.01 EU/μg

Immunogen: Recombinant Murine TNF alpha expressed in E. coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA: 1 μg/mL - 10 μg/mL. Functional Assays. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 without preservatives., None, None

Konservierungsmittel: Without preservative

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C. DO NOT FREEZE!

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor (TNF-Alpha)

Hintergrund: TNF alpha is a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immuno-regulatory role.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

Gen-ID: 10090

UniProt: P06804

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome