TLR4 Antikörper

Produktnummer ABIN119330

Der Maus Monoklonal Anti-TLR4 Antikörper wurde für WB, FACS, IP und Func validiert. Er ist geeignet, TLR4 in Proben von Human, Hund, Schwein, Meerschweinchen und Affe zu detektieren.

Kurzübersicht für TLR4 Antikörper (ABIN119330)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reaktivität: Human, Hund, Schwein, Meerschweinchen, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TLR4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Klon: HTA125

Produktdetails anti-TLR4 Antikörper

Aufreinigung: Affinity chromatography on Protein G

Endotoxin-Niveau: < 0.01 EU/ug

Immunogen: Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunoprecipitation. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, None, None

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Andere Bezeichnung: CD284 / TLR4

Hintergrund: TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 Molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.Synonyms: Toll-like receptor 4

NCBI Accession: NM_138554

UniProt: O00206

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteine