CD11b Antikörper

Produktnummer ABIN118517

Der Maus Monoklonal Anti-CD11b-Antikörper wurde für FACS, IP, IHC (fro) und Func validiert. Er ist geeignet, CD11b in Proben von Human und Affe zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für CD11b Antikörper (ABIN118517)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD11b Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Klon: ICRF44

Produktdetails anti-CD11b Antikörper

Spezifität: This antibody recognises the CD11b cell surface glycoprotein. This antibody blocks some functions of CD11b, although full definition is still to be completed.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Rheumatoid synovial cells and human mocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 10^6 cells or 100 μL whole blood. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-CD11b Antikörper (ABIN118517)

Sotiriou, Orlova, Al-Fakhri, Ihanus, Economopoulou, Isermann, Bdeir, Nawroth, Preissner, Gahmberg, Koschinsky, Chavakis: "Lipoprotein(a) in atherosclerotic plaques recruits inflammatory cells through interaction with Mac-1 integrin." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 559-61, (2006) (PubMed).

Details zu CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung: CD11b

Hintergrund: CD11b is a 165kD molecule also known as the alphaM integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gen-ID: 3684

NCBI Accession: NP_000623

UniProt: P11215

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation