CD14 Antikörper

Produktnummer ABIN118508

Der Maus Monoklonal anti-CD14 Antikörper wird verwendet zum Nachweis von CD14 in Proben von Human, Affe, Schwein, Hund, Rind (Kuh), Schaf, Kaninchen, Ziege, Cat und Nerz. Er wurde validiert für FACS, WB, Func und EIA.

Kurzübersicht für CD14 Antikörper (ABIN118508)

Target: CD14

Reaktivität: Human, Affe, Schwein, Hund, Rind (Kuh), Schaf, Kaninchen, Ziege, Cat, Nerz

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Klon: Tük4

Produktdetails anti-CD14 Antikörper

Aufreinigung: Affinity chromatography on Protein G

Endotoxin-Niveau: Low Endotoxin

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/100 - 1/200, use 10 μL of suggested working dilution to label 1x10^6 cells or 100 μL whole blood. ELISA. Western Blot: under reducing conditions. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Gen-ID: 9606

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades