CD14
Reaktivität: Human
WB, IHC
Wirt: Maus
Monoclonal
LPSR-2397
unconjugated
Applikationshinweise
Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells or 100 μL whole blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.1 mg/mL
Buffer
PBS, 0.09 % Sodium Azide, 1 % BSA
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Brückner, Tautenhahn, Winkler, Stock, Dollinger, Christ: "A fat option for the pig: hepatocytic differentiated mesenchymal stem cells for translational research." in: Experimental cell research, Vol. 321, Issue 2, pp. 267-75, (2014) (PubMed).
Brückner, Tautenhahn, Winkler, Stock, Jonas, Dollinger, Christ: "Isolation and hepatocyte differentiation of mesenchymal stem cells from porcine bone marrow--"surgical waste" as a novel MSC source." in: Transplantation proceedings, Vol. 45, Issue 5, pp. 2056-8, (2013) (PubMed).
Target
CD14
Andere Bezeichnung
CD14
Hintergrund
CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein