CD40 Antikörper (Extracellular Domain) (PE)

Produktnummer ABIN118124

Der Ratte Monoklonal anti-CD40 Antikörper wird verwendet zum Nachweis von CD40 in Proben von Maus. Er wurde validiert für FACS.

Kurzübersicht für CD40 Antikörper (Extracellular Domain) (PE) (ABIN118124)

Target: CD40

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD40 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: 01-03-23

Produktdetails anti-CD40 Antikörper (PE)

Bindungsspezifität: Extracellular Domain

Spezifität: This antibody recognises CD40 and stains most mature mouse B cells. T cells are unlabelled.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1. Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with distilled water: SM008R with 1.0 ml. SM008RT with 0.25 mL SM008RX with 5.0 ml

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu CD40

Target: CD40

Andere Bezeichnung: CD40

Hintergrund: CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5

Gen-ID: 21939

NCBI Accession: NP_035741

UniProt: P27512

Pathways: NF-kappaB Signalweg, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints