TNF alpha Antikörper

Produktnummer ABIN118027

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch TNF alpha in WB, IP, IHC (p), Func, EIA, IHC (fro) und RIA. Er zeigt eine Reaktivität gegenüber Maus und wurde in 1 Publikation erwähnt.

Kurzübersicht für TNF alpha Antikörper (ABIN118027)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Produktdetails anti-TNF alpha Antikörper

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Selective salt precipitation

Immunogen: Recombinant Mouse TNF-alpha produced in E.coli.

Anwendungsinformationen

Applikationshinweise: Western Blot: Use HRP Goat-anti-Rabbit IgG [H&L] (code R1364HRP) and TMB as asubstrate. A working dilution range of 1: 500 to 1: 2,000 is suggested for this application to detect TNFαfrom supernatants or lysates of 2 x 10^6 endotoxin-stimulated mouse peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) mouse serumplus 10 ng/mL E. coli LPS. Immunohistochemistry: A dilution range of 1: 400 is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry using adilution of 1: 200 for staining of TNFα.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C.

Referenzen für anti-TNF alpha Antikörper (ABIN118027)

Liu, Zhang, Chen, Wang, Qiu, Liu, Wan, Guo: "Effects and mechanisms of alveolar type II epithelial cell apoptosis in severe pancreatitis-induced acute lung injury." in: Experimental and therapeutic medicine, Vol. 7, Issue 3, pp. 565-572, (2014) (PubMed).

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor (TNF-Alpha)

Hintergrund: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

Gen-ID: 10090

UniProt: P06804

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome