GSK3 alpha Antikörper (pSer21)

Produktnummer ABIN117908

Produktdetails anti-GSK3 alpha Antikörper

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Bindungsspezifität: AA 16-25, pSer21

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GSK3 alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequenz: Protein Sequence: Human GSK3 alpha, 483 aa, predicted MW 51.0 kDa 1 msgggpsggg pggsgrarts sfaepggggg gggggpggsa sgpggtgggk asvgamgggv 61 gasssgggpg gsggggsggp gagtsfpppg vklgrdsgkv ttvvatlgqg persqevayt 121 dikvigngsf gvvyqarlae trelvaikkv lqdkrfknre lqimrkldhc nivrlryffy 181 ssgekkdely lnlvleyvpe tvyrvarhft kakltipily vkvymyqlfr slayihsqgv 241 chrdikpqnl lvdpdtavlk lcdfgsakql vrgepnvsyi csryyrapel ifgatdytss 301 idvwsagcvl aelllgqpif pgdsgvdqlv eiikvlgtpt reqiremnpn ytefkfpqik 361 ahpwtkvfks rtppeaialc sslleytpss rlspleacah sffdelrclg tqlpnnrplp 421 plfnfsagel siqpslnail ipphlrspag tttltpssqa ltetptssdw qstdatptlt 481 nss

Spezifität: This affinity-purified antibody is directed against the phosphorylated form of human GSK3A at the pS 21 residue. The product was affinity purified from monospecific antiserum by Immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody reacts with phosphorylated pS21 of human GSK3A. Reactivity with non-phosphorylated human GSK3A is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and rat based on 100 % homology for the immunogen sequence. Cross reactivity with GSK3A homologues from other sources has not been determined.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 16-25 of human GSK3 alpha.

Anwendungsinformationen

Applikationshinweise: This affinity-purified antibody has been tested for use in ELISA (1: 2,000-1: 10,000) and byWestern blot (1: 500-1: 3,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 52 kDa in size corresponding to GSK3A by Westernblotting in the appropriate cell lysate or extract. Less than 1 % reactivity is observed against the non-phosphorylated form of theimmunizing peptide. This antibody is phosphor specific for pS21 of GSK3A.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Referenzen für anti-GSK3 alpha Antikörper (ABIN117908)

Wang, He, Wang, Wang, Zhai, Zhou, Zhang, Zhai, Feng, Han: "[Impact of Donor KIR2DS5 Genotype on Outcome Following Haploidentical Hematopoietic Stem Cell Transplantation.]" in: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 16, Issue 1, pp. 111-5, (2008) (PubMed).

Details zu GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Andere Bezeichnung: GSK3 alpha (GSK3a Produkte)

Synonyme: 2700086H06Rik antikoerper, GSK3A antikoerper, GSK-3[a] antikoerper, GSK-3[b] antikoerper, GSK3 antikoerper, gsk3a antikoerper, xgsk3 antikoerper, glycogen synthase kinase 3 alpha antikoerper, glycogen synthase kinase 3 alpha b antikoerper, glycogen synthase kinase 3 alpha S homeolog antikoerper, GSK3A antikoerper, Gsk3a antikoerper, gsk3a antikoerper, gsk3ab antikoerper, gsk3a.S antikoerper, Bm1_31380 antikoerper

Hintergrund: Glycogen synthase kinase 3 alpha (GSK3A) belongs to the ser/thr family of protein kinases, Cdc2/cdkx subfamily, gsk-3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c-jun. GSK3A is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A) and beta (GSK3B), show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha

Gen-ID: 2931

NCBI Accession: NP_063937

UniProt: P49840

Pathways: PI3K-Akt Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effekt