TNF alpha Antikörper

Produktnummer ABIN1177286

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-TNF alpha Antikörper wird verwendet zum Nachweis von TNF alpha in Proben von Human. Er wurde validiert für ELISA und Neut.

Kurzübersicht für TNF alpha Antikörper (ABIN1177286)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: ELISA, Neutralization (Neut)

Klon: MABTNF-A5

Produktdetails anti-TNF alpha Antikörper

Produktmerkmale: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human TNF. Neutralization activity may be measured using a cytolysis assay for MTT dye conversion with 2.0 ng/mL recombinant human TNF (Cat. No. 554618) and L929 at 2 x 10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human TNF for 60 minutes).
50% Neutralization (ND50) at 1 -100 ng/ml
>90% Neutralization at 50-300 ng/mL

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Recombinant human TNF protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-TNF alpha Antikörper (ABIN1177286)

Petyovka, Lyach, Voitenok: "Homologous ELISA for detection of oligomeric human TNF: properties of the assay." in: Journal of immunological methods, Vol. 186, Issue 2, pp. 161-70, (1995) (PubMed).

Kriegler, Perez, DeFay, Albert, Lu: "A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: ramifications for the complex physiology of TNF." in: Cell, Vol. 53, Issue 1, pp. 45-53, (1988) (PubMed).

Smith, Baglioni: "The active form of tumor necrosis factor is a trimer." in: The Journal of biological chemistry, Vol. 262, Issue 15, pp. 6951-4, (1987) (PubMed).

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF

Hintergrund: The MABTNF-A5 antibody, formerly known as 5N, reacts with human tumor necrosis factor, TNF (aka, TNF-alpha and TNFSF2). TNF is a multifunctional cytokine involved in a variety of immune and inflammatory responses including hemorrhagic necrosis of tumors, septic shock, fever and autoimmune diseases. TNF can regulate the growth and differentiation of many different cell types. It is produced by different activated cell types including macrophages, T lymphocytes, NK cells, dendritic cells, endothelial cells, peripheral blood leukocytes, osteoblasts, astrocytes, mast cells, Kupffer cells, smooth muscle cells, fibroblasts and certain tumor cells. TNF exists in two biologically active forms, i.e., transmembrane and soluble forms. Upon activation, cells express transmembrane TNF glycoproteins that associate as homotrimeric complexes. After enzymatic cleavage, the extracellular region of membrane TNF sheds as a soluble homotrimer. The biologically active form of TNF has been reported to be a trimer. The immunogen used to generate the MABTNF-A5 hybridoma was purified recombinant human TNF. This is an extraordinarily potent neutralizing antibody that is reportedly also useful for ELISA measurements of human TNF.

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome