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IRAK4 Antikörper (PE)

IRAK4 Reaktivität: Human ICS Wirt: Maus Monoclonal L29-525 PE
Produktnummer ABIN1176904
  • Target Alle IRAK4 Antikörper anzeigen
    IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))
    Reaktivität
    • 116
    • 67
    • 27
    • 16
    • 14
    • 13
    • 3
    • 3
    • 2
    • 2
    • 1
    Human
    Wirt
    • 109
    • 12
    • 1
    Maus
    Klonalität
    • 108
    • 14
    Monoklonal
    Konjugat
    • 53
    • 8
    • 7
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    Dieser IRAK4 Antikörper ist konjugiert mit PE
    Applikation
    • 112
    • 52
    • 37
    • 26
    • 18
    • 16
    • 15
    • 10
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Intracellular Staining (ICS)
    Marke
    BD Phosflow™
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human IRAK4 Recombinant Protein
    Klon
    L29-525
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product IRAK4 Primärantikörper
  • Applikationshinweise
    This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells and cell lines (using BD™ Phosflow Lyse/Fix Buffer or BD Cytofix™ Fixation Buffer), but not human whole blood. BD™ Phosflow Perm Buffer II or Perm Buffer III may be used.
    Probenmenge
    20 μL
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. Confirmation of the specificity of mAb L29-525 by western blot analysis (left panel). Lysates of human PBMC were probed with unconjugated antibody at a concentration of 0.1 μg/ml. IRAK4 is identified as a band of about 52 kDa. Western blot validation of IRAK4 by RNAi (right panel). Lysates from HeLa S3 cells (lane 1) and IRAK4 RNAi-transfected HeLa S3 cells (lane 2) were probed with unconjugated mAb L29-525 at 0.015 μg/ml. Down-regulation of IRAK4 expression is evident in the RNAi-transfected cells.
  • Suzuki, Saito: "IRAK-4--a shared NF-kappaB activator in innate and acquired immunity." in: Trends in immunology, Vol. 27, Issue 12, pp. 566-72, (2006) (PubMed).

    Hatao, Muroi, Hiki, Ogawa, Mimura, Kaminishi, Tanamoto: "Prolonged Toll-like receptor stimulation leads to down-regulation of IRAK-4 protein." in: Journal of leukocyte biology, Vol. 76, Issue 4, pp. 904-8, (2004) (PubMed).

    Lye, Mirtsos, Suzuki, Suzuki, Yeh: "The role of interleukin 1 receptor-associated kinase-4 (IRAK-4) kinase activity in IRAK-4-mediated signaling." in: The Journal of biological chemistry, Vol. 279, Issue 39, pp. 40653-8, (2004) (PubMed).

    Li, Strelow, Fontana, Wesche: "IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 8, pp. 5567-72, (2002) (PubMed).

  • Target
    IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))
    Andere Bezeichnung
    IRAK4 (IRAK4 Produkte)
    Synonyme
    zgc:55553 antikoerper, IRAK4 antikoerper, ipd1 antikoerper, irak-4 antikoerper, ny-ran-64 antikoerper, ny-ren-64 antikoerper, ren64 antikoerper, irak4 antikoerper, IPD1 antikoerper, IRAK-4 antikoerper, NY-REN-64 antikoerper, REN64 antikoerper, 8430405M07Rik antikoerper, 9330209D03Rik antikoerper, interleukin 1 receptor associated kinase 4 antikoerper, interleukin-1 receptor-associated kinase 4 antikoerper, IRAK4 antikoerper, irak4 antikoerper, Irak4 antikoerper
    Hintergrund
    Interleukin 1 (IL-1) is a proinflammatory cytokine that mediates the host inflammatory response. IL-1 exerts its biological effects through its type I receptor (IL-1RI), which is found on most cell types. When IL-1 binds to the IL-1RI, NF-kappaB (transcription factor) is activated and translocates to the nucleus where it induces gene expression. IL-1-receptor-associated kinase (IRAK) has been reported to co-immunoprecipitate with IL-1RI. The IRAK family is made up of four identified Pelle homologs that regulate the activation of NF-kappaB and mitogen-activated protein (MAP) kinase pathways. IRAK4 is a unique member of the IRAK family because it is the closest mammalian homolog to Pelle. Also, when overexpressed, its kinase activity allows it to activate both the NF-kappaB and mitogen-activated protein (MAP) kinase pathways, and block the IL-1-induced activation and modification of IRAK1. IRAK4 is also able to phosphorylate IRAK1, which suggests that IRAK4 affects the early signal transduction of Toll/IL-1 receptors before the involvement of IRAK1. The L29-525 monoclonal antibody recognizes human IRAK4, regardless of phosphorylation status. The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure). Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.
    Synonyms: IRAK-4, REN64, Q69FE3
    Pathways
    NF-kappaB Signalweg, TLR Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades
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