TNF alpha Antikörper (Biotin)

Produktnummer ABIN116241

Der Biotin-konjugierte Kaninchen Polyklonal anti-TNF alpha Antikörper (ABIN116241) detektiert spezifisch TNF alpha in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TNF alpha Antikörper (Biotin) (ABIN116241)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-TNF alpha Antikörper (Biotin)

Aufreinigung: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Human TNF-Alpha (Cat.-No PA132)

Anwendungsinformationen

Applikationshinweise: ElisaDirect: Using 100 μL/well antibody solution, a concentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 -0.4 ng/well of recombinant hTNF-α. Sandwich: Using 100 μL/well antibody solution, a concentration of 0.25 - 1.0 μg/mL isrequired. In conjunction with polyclonal Anti-Human TNF-α as a capture antibody, it allowsthe detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-α. Western Blot1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody can be stored at -20 °C. Following reconstitution, store at 2-8 °C for up to two weeks or at -20 °C for up to six months.

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor (TNF-Alpha)

Hintergrund: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

Gen-ID: 9606

UniProt: P01375

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome