IL-6 Antikörper (Biotin)

Produktnummer ABIN116082

Der Biotin-konjugierte Ziege Polyklonal anti-IL-6 Antikörper (ABIN116082) detektiert spezifisch IL-6 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für IL-6 Antikörper (Biotin) (ABIN116082)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IL-6 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-IL-6 Antikörper (Biotin)

Spezifität: This antibody detects Interleukin-6.

Aufreinigung: Affinity chromatograophy

Immunogen: Highly pure (> 98 %) recombinant human IL-6

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect hIL-6 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Sandwich: To detect hIL-6 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-Human IL-6as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinanthIL-6. Western blot: To detect hIL-6 this antibody can be used at a concentration of 0.1 - 0.2g/mL. Used in conjunction with compatible secondary reagents the detection limit for

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Andere Bezeichnung: Interleukin-6 (IL6)

Hintergrund: Interleukin 6 (IL6) is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass. IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.Synonyms: B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFNB2, IL-6, Interferon beta-2

Gen-ID: 3569

NCBI Accession: NP_000591

UniProt: P05231

Pathways: TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome