TNF alpha Antikörper

Produktnummer ABIN115973

Der Kaninchen Polyklonal anti-TNF alpha Antikörper wird verwendet zum Nachweis von TNF alpha in Proben von Ratte. Er wurde validiert für WB, Func und EIA.

Kurzübersicht für TNF alpha Antikörper (ABIN115973)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Produktdetails anti-TNF alpha Antikörper

Spezifität: This antibody detects TNF-alpha.

Aufreinigung: Affinity Chromatography employing immobilized Rat TNF-a matrix

Immunogen: Highly pure (>98%) recombinant Rat TNF-alpha

Anwendungsinformationen

Applikationshinweise: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of Rat TNF-alpha(2.0 ng/mL), a concentration of 0.07 - 0.1 μg/mL of this antibody is required. ELISAIndirect: To detect Rat TNF-alpha by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant Rat TNF-alpha. Sandwich: To detect Rat TNF-alpha by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, inconjunction with biotinylated Anti-Rat TNF-alpha (PP039B1 or PP039B2) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-alpha. Western BlotTo detect Rat TNF-alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Rat TNF-alpha is 1.5 - 3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge the vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor (TNF-Alpha)

Hintergrund: TNF-alpha is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

Gen-ID: 24835

NCBI Accession: NP_036807

UniProt: P16599

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome