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Collagen IV Antikörper

COL4 Reaktivität: Maus, Orang-Utan IHC (p), IF, EIA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN114114
  • Target Alle Collagen IV (COL4) Antikörper anzeigen
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Reaktivität
    • 88
    • 42
    • 40
    • 23
    • 16
    • 10
    • 5
    • 1
    Maus, Orang-Utan
    Wirt
    • 56
    • 26
    • 12
    Kaninchen
    Klonalität
    • 67
    • 27
    Polyklonal
    Konjugat
    • 44
    • 10
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Dieser Collagen IV Antikörper ist unkonjugiert
    Applikation
    • 61
    • 45
    • 30
    • 29
    • 27
    • 26
    • 26
    • 23
    • 16
    • 9
    • 8
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
    Spezifität
    Antibody BP975 is specific for Mouse collagen type IV. The following cross reactivities have been observed: Mouse Type IV: 100 % Mouse Type III: < 0.1 % Mouse Type II: < 0.1 % Mouse Type I: < 0.1 % Human Type V: < 0.1 % Human Type IV: < 0.1 % Mouse Fibronectin: < 0.1 % Mouse Laminin: < 0.1 %
    Kreuzreaktivität (Details)
    Species reactivity (expected):OrangutanSpecies reactivity (tested):Mouse
    Aufreinigung
    Antigen Affinity Chromatography
    Immunogen
    Collagen IV purified from Mouse EHS tumour
    Top Product
    Discover our top product COL4 Primärantikörper
  • Applikationshinweise
    Immunohistochemistry on Paraffin Embedded Sections (1/250-1/1,000): Antigen retrievalis not necessary but may improve results, the recommended fixatives are AFA, 5-10 % buffered formalin or Bouin's. Immunofluorescence (1/40-1/60). ELISA (1/100-1/400).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer
    PBS, 0.09 % Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store undiluted (in aliquots) at -20 °C.
  • Target
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Andere Bezeichnung
    Collagen Type IV (COL4 Produkte)
    Synonyme
    collagen, type IV antikoerper, Let-2 antikoerper
    Hintergrund
    Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.Synonyms: COL4A1
    Gen-ID
    12826
    NCBI Accession
    NP_034061
    UniProt
    P02463
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