Collagen IV Antikörper

Produktnummer ABIN114114

Dieses Anti-Collagen IV-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Collagen IV in IHC (p), IF und EIA. Geeignet für Maus und Orang-Utan.

Kurzübersicht für Collagen IV Antikörper (ABIN114114)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Maus, Orang-Utan

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-Collagen IV Antikörper

Spezifität: Antibody BP975 is specific for Mouse collagen type IV. The following cross reactivities have been observed: Mouse Type IV: 100 % Mouse Type III: < 0.1 % Mouse Type II: < 0.1 % Mouse Type I: < 0.1 % Human Type V: < 0.1 % Human Type IV: < 0.1 % Mouse Fibronectin: < 0.1 % Mouse Laminin: < 0.1 %

Kreuzreaktivität (Details): Species reactivity (expected):OrangutanSpecies reactivity (tested):Mouse

Aufreinigung: Antigen Affinity Chromatography

Immunogen: Collagen IV purified from Mouse EHS tumour

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Paraffin Embedded Sections (1/250-1/1,000): Antigen retrievalis not necessary but may improve results, the recommended fixatives are AFA, 5-10 % buffered formalin or Bouin's. Immunofluorescence (1/40-1/60). ELISA (1/100-1/400).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted (in aliquots) at -20 °C.

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen Type IV

Hintergrund: Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.Synonyms: COL4A1

Gen-ID: 12826

NCBI Accession: NP_034061

UniProt: P02463