Collagen IV Antikörper

Produktnummer ABIN113972

Der Kaninchen Polyklonal Anti-Collagen IV-Antikörper wurde für IHC (fro), IHC (p), IF, EIA und RIA validiert. Er ist geeignet, Collagen IV in Proben von Schwein zu detektieren.

Kurzübersicht für Collagen IV Antikörper (ABIN113972)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Produktdetails anti-Collagen IV Antikörper

Spezifität: This antibody is specific for Collagen type IV (100 %). Porcine Collagen type I, II, III, Elastin, Fibronectin and Laminin < 0.1 % (in RIA at 1/1000 dilution).

Aufreinigung: Affinity Chromatography.

Immunogen: Purified collagen type IV from porcine renals.

Anwendungsinformationen

Applikationshinweise: ELISA: > 1/200 gives OD > 500. RIA. Immunofluorescence (1/80). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/500. Incubation time: 60 min. at RT or 2-8 °C overnight. Positive Control: Porcine renals. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water. For further dilution use appropriate antibody diluent.

Konzentration: 1.0 mg/mL

Buffer: PBS without BSA and preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen Type IV

Hintergrund: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type IV Collagen (170-180 kDa), is a non-fibrilary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, renals).Synonyms: COL4A1

Gen-ID: 1282

NCBI Accession: NP_001836

UniProt: P02462