IL4 Receptor Antikörper (Extracellular Domain)

Produktnummer ABIN112606

Produktdetails anti-IL4 Receptor Antikörper

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Bindungsspezifität: Extracellular Domain

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL4 Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Spezifität: This antibody is specific for Interleukin 4 Receptor, the transmembrane ligand-binding subunit of the heterodimeric IL-4 receptor complex. This antibody blocks cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/mL in the presence of 0.2 ng/mL of IL-4 using the factor dependant Human erythroleukemic cell line, TF-1.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography on Protein A

Immunogen: Sf 21-derived, recombinant Human interleukin 4 Receptor, extracellular domain.

Klon: 25463 (49-20)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/20 - 1/80. ELISA: 1/500 - 1/2000. Western Blot: 1/250 - 1/1000, Will detect human IL-4 R under non-reducing conditions. Functional Assays. Immunohistochemistry on frozen sections: 1/40 - 1/125.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 10 % Trehalose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Andere Bezeichnung: CD124 / IL4R (IL4R Produkte)

Hintergrund: IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation. The IL4 response is involved in promoting Th2 differentiation. The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. It has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.Synonyms: 582J2.1, IL-4R-alpha, IL4 Receptor alpha, IL4RA, Interleukin-4 receptor alpha chain

Gen-ID: 3566

NCBI Accession: NP_000409

UniProt: P24394

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process