CD14 Antikörper

Produktnummer ABIN112001

Der Maus Monoklonal anti-CD14 Antikörper wird verwendet zum Nachweis von CD14 in Proben von Human. Er wurde validiert für FACS und IHC (fro).

Kurzübersicht für CD14 Antikörper (ABIN112001)

Target: CD14

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: Mab0211

Produktdetails anti-CD14 Antikörper

Aufreinigung: Affinity chromatography.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemitry (IHC) on frozen sections: 1 μg/mL (1: 200)and Facs. Antigen Distribution: Isolated cells: Positive on granulocytes and monocytes, myeloid leukemias and myeloidcell lines. Tissue sections: Positive on histocytes, Langerhans cells, follicular dendritic cells and highendothelial venules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum For further information and details see technicalinformation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water

Buffer: Phosphate buffered saline, pH 7.2 with 0.02 % sodium azide as preservative and 1 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: CD14 is a 53'000 - 55'000 Mr glycoprotein expressed strongly on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Gen-ID: 9606

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades